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KMID : 0613820010110040311
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Journal of Life Science
2001 Volume.11 No. 4 p.311 ~ p.315
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Effects of Embryo Development Stage and Gelrite Concentration on Plant Regeneration in Seed Culture of Rice
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Kwon Yong-Sham
Kim Kyung-Min
Kim Do-Hoon
Son Jae-Keun
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Abstract
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To identify the effects embryo developmental stage and gelrite concentration on plant regeneration in seed culture of rice, mature and immature seeds of rice were cultured on the N_{6} medium supplemented with2 mg/ell 2.4-D and different levels of gelrite(0.2~1.0%). The calli formed immature embryos were produced more plants than those from mature embryos. The maximum frequency of plant regeneration was achieved in the culture of the calli of immature embryos which was harvested at the 21^{th} day after pollination. The plant regeneration on the medium with gelrite was more accelerate than that on the medium with agar. The highest frequency(55%) of plant regeneration was obtained from the calli transferred to the medium with 6g/ell gelrite.
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KEYWORD
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Rice, Seed culture, Immature embryo, Gelrite
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